A Twist in the Tale of a Pig Model of Short-Bowel Syndrome

hort-bowel syndrome (SBS) occurs after a long surSgical resection of small intestine. This results in malabsorption of nutrients, especially lipids, and is associated with the development of liver disease. It is particularly serious in infants. The contributions of altered lipid metabolism, bile acid physiology, dysbiosis, and supplemental enteral or parenteral nutrition to the development of this pathology remain unclear, and a piglet model has provided useful evidence due to various similarities with humans. In the study of SBS-induced liver damage by PereiraFantini et al published in this issue of Cellular and Molecular Gastroenterology and Hepatology, piglets had resection of 75% of the small intestine, leaving duodenum and terminal ileum in situ, or a sham operation. Animals were fed with oral formula and were studied 2 weeks postsurgery when liver, terminal ileum, bile, and portal blood samples were collected. Half the piglets in each group were given the farnesoid X receptor (FXR) agonist, obeticholic acid (OCA). This is a semisynthetic bile acid, with an additional 6-alpha-ethyl group, which increases its potency at the FXR, in mouse and humans at least, by almost 100-fold. OCA has been shown to be beneficial in many animal models of cholestasis and steatosis, and in clinical trials for primary biliary cholangitis, nonalcoholic steatohepatitis, and primary bile acid diarrhea. OCA was a plausible drug to study as previous work by the same authors had suggested impaired FXR responses in SBS piglets. Also, in the parenteral nutrition–fed piglet, the most potent natural bile acid FXR agonist, chenodeoxycholic acid, improved the liver disease. As expected, in the SBS model weight gain was reduced, feces were unformed and fatty (steatorrhea), and liver changes including lipid droplets and clusters of inflammatory cells were found. OCA treatment reduced stool fat to a large extent, improving absorption. SBS was associated with depletion of biliary taurine and OCA reversed this. There were many changes in the composition of bile acids in the bile in SBS, in particular a large increase in hyocholic acid, which is a major primary bile acid in the pig. There were major decreases in SBS of the secondary bile acids, which result from intestinal bacterial metabolism. OCA treatment partially restored these conjugated secondary bile acids in bile. The rate-limiting gene for bile acid synthesis by the classical pathway in the liver is CYP7A1, and expression of this was potently inhibited by OCA in control and SBS pigs, as reported in other species. OCA up-regulated several other known FXR-responsive genes in the liver and there were only minor differences between its effects in control or SBS animals. However, there were several unexpected findings. In the SBS animals, OCA resulted in more fat accumulation in the liver and a significant reduction in lobule size. Intestinal FXR